To calculate the concentration of DNA in a solution based on absorbance, we typically use the following formula derived from Beer's Law:
\[ \text{Concentration} = \frac{\text{Absorbance}}{\text{Path Length} \times \text{Quadratic Coefficient}} \]
For DNA, the common absorbance values that we use (depending on the context) are typically:
- For dsDNA at 260 nm, a typical value is 50 µg/mL per absorbance unit (1 OD).
Assuming the path length is 1 cm (standard for most cuvettes), we can calculate the concentration using the absorbance value provided:
\[ \text{Concentration} = \text{Absorbance} \times 50 , \text{µg/mL} \]
Given the corrected absorbance is 2.49:
\[ \text{Concentration} = 2.49 \times 50 , \text{µg/mL} = 124.5 , \text{µg/mL} \]
Therefore, rounded to one decimal place, the concentration of DNA in the solution is:
Answer: 124.5 µg/mL
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