What is the concentration of DNA in a solution if the corrected absorbance is 2.49?

You may copy/paste the following to indicate micro- μ. Report your answer to one decimal place.



Your Answer:
Question 14 options:
Answer:
units:

1 answer

To calculate the concentration of DNA in a solution based on absorbance, we typically use the following formula derived from Beer's Law:

\[ \text{Concentration} = \frac{\text{Absorbance}}{\text{Path Length} \times \text{Quadratic Coefficient}} \]

For DNA, the common absorbance values that we use (depending on the context) are typically:

  • For dsDNA at 260 nm, a typical value is 50 µg/mL per absorbance unit (1 OD).

Assuming the path length is 1 cm (standard for most cuvettes), we can calculate the concentration using the absorbance value provided:

\[ \text{Concentration} = \text{Absorbance} \times 50 , \text{µg/mL} \]

Given the corrected absorbance is 2.49:

\[ \text{Concentration} = 2.49 \times 50 , \text{µg/mL} = 124.5 , \text{µg/mL} \]

Therefore, rounded to one decimal place, the concentration of DNA in the solution is:

Answer: 124.5 µg/mL