The general equation used to determine the concentration of a biological molecule in solution from its corrected absorbance is based on Beer's Law (also known as Beer-Lambert Law). The equation is typically written as:
\[ A = \epsilon \cdot c \cdot l \]
Where:
- \( A \) is the corrected absorbance.
- \( \epsilon \) is the molar absorptivity (extinction coefficient) of the molecule (in units of L/(mol·cm)).
- \( c \) is the concentration of the molecule in solution (in units of mol/L or M).
- \( l \) is the path length of the cuvette or sample container (in cm).
To solve for the concentration \( c \), the equation can be rearranged as:
\[ c = \frac{A}{\epsilon \cdot l} \]
This equation equates the corrected absorbance to the product of molar absorptivity and concentration times the path length, allowing you to calculate the concentration of the molecule in solution.