You determine the corrected absorbance of your DNA solution at two different wavelengths to be: A260 =1.88 and A280=0.99 What is the concentration of DNA in your sample?

Question

You may copy/paste the following to indicate "micro"- μ. Report your answer to one decimal place.

Answer 1

To calculate the concentration of DNA based on the absorbance readings at 260 nm (A260) and 280 nm (A280), you can use the following formula:

\[ \text{Concentration (μg/mL)} = A260 \times 50 \]

Given your A260 reading of 1.88:

\[ \text{Concentration (μg/mL)} = 1.88 \times 50 = 94.0 , μg/mL \]

Thus, the concentration of DNA in your sample is 94.0 μg/mL.