You determine the corrected absorbance of your DNA solution at two different wavelengths to be: A260 =1.88 and A280=0.99 What is the concentration of DNA in your sample?

Question

You may copy/paste the following to indicate "micro"- μ. Report your answer to one decimal place.

Be sure that your work:

states the A260 provided
states the A280 provided
identifies the equation that you used to solve the problem,
identifies which numbers you substituted for which variables in the equation,
exhibits the math necessary to solve for the answer,
and includes appropriate units with your answer,
You may copy/paste the following to indicate "micro"- μ
Hint: An equation "equates" two entities. If you do not include two things separated by an "=", then you have not written an equation -- you will have simply written a mathematical operation.

Answer 1

To determine the concentration of DNA in the given sample based on the absorbance readings, we will use the following information:

• A260 provided: 1.88
• A280 provided: 0.99

The equation to calculate the DNA concentration in micrograms per milliliter (μg/mL) is:

\[ \text{Concentration (μg/mL)} = A260 \times 50 \]

This equation is based on the fact that an A260 of 1.0 corresponds to approximately 50 μg/mL of double-stranded DNA.

Now, substituting the provided A260 value into the equation:

\[ \text{Concentration (μg/mL)} = 1.88 \times 50 \]

Performing the multiplication gives us:

\[ \text{Concentration (μg/mL)} = 94.0 \]

Thus, the concentration of DNA in the sample is 94.0 μg/mL.