For High Performance Liquid Chromatography (i.e. HPLC), can the column be flushed with Isopropanol (i.e. IPA)?

1 answer

It will depend on the column's stationary phase. After flushing the column with IPA this should then be replaced with the mobile phase that is going to be used. Some of the more complex stationary phases do not take kindly to IPA.

(Glad you defined what you meant by IPA, I once had a student who used IPA (=beer) to develop a TLC plate!)
Similar Questions
    1. answers icon 1 answer
  1. Hi, I have a question on HPLC (high performance liquid chromatography).The procedure says to use a 4.6 x 150mm x 3um column. The
    1. answers icon 1 answer
  2. Hi,I have a question on column length for HPLC (high performance liquid chromatography). If the column length is increased,eg
    1. answers icon 1 answer
  3. I have a method using HPLC (high performance liquid chromatography).It states that a 4.6 x 150mm,3um column should be used.
    1. answers icon 0 answers
more similar questions