Question
Hi,
I have a question on column length for HPLC (high performance liquid chromatography).
If the column length is increased,eg from 100mm to 150mm and all other column parameters e.g. particle size of stationary phase, internal diameter..etc.remain the same, how does it affect resolution of peaks? Do I expect better resolution?
I have a question on column length for HPLC (high performance liquid chromatography).
If the column length is increased,eg from 100mm to 150mm and all other column parameters e.g. particle size of stationary phase, internal diameter..etc.remain the same, how does it affect resolution of peaks? Do I expect better resolution?
Answers
bobpursley
Yes, better resolution as the time delay between peaks is longer
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