Question
I have a new column for HPLC (high-performance liquid chromatography) analysis.
Is it advisable to condition the new column with the mobile phase overnight at a slow flow rate of 0.5ml/min?
Is it advisable to condition the new column with the mobile phase overnight at a slow flow rate of 0.5ml/min?
Answers
Frankly, I don't know if that is required; however, I think it's always wise to do so.
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