Question

How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is 121, mw of Hcl is 157.

Answer 1

Pka is Tn3 =8.3
MW of Tn3 =121
MW of Tn3 HCL=157

Answer 2

From the question given: Tris Hcl us the Acid,with Mw=157.and then Tris is the base with the Mw=121. then converts 500mls to litre which is 0.5 litre then from the Henderson and Hasselbach equation PH=pka+log(A/H) then 8.0-8.3=log Tri/Tri Hcl then -0.3=log(Tri/Trid HCl =0.50 from antilog of -0.3= Tris/TrisHCl ......... Loading