Asked by seth
how many grams of tris and tris Hcl are needed to make up 500ml of 1m tris buffer with a pH of 0.8?
Answers
Answered by
DrBob222
I assume you mean 1 M and not 1 m. M is molar; m is molal.
You need two equations.
equation 1 is
pH = pKa + log (base)/(acid)
equation 2 is
(base) + (acid) = 0.8 M
Use equation 1 and solve for (base)/(acid) then (base) = k(acid). Then plug that into equation 1 to find (base) and (acid). Convert molarities into grams per liter , then grams/500 mL.
Post your work if you get stuck.
You need two equations.
equation 1 is
pH = pKa + log (base)/(acid)
equation 2 is
(base) + (acid) = 0.8 M
Use equation 1 and solve for (base)/(acid) then (base) = k(acid). Then plug that into equation 1 to find (base) and (acid). Convert molarities into grams per liter , then grams/500 mL.
Post your work if you get stuck.
Answered by
Anonymous
Question makes no sense. pH of 0.8 or 8.0????
Answered by
Ojole Joseph
pH is 8.0
Answered by
Doc48
Here's a reference that's useful for tris systems...(remove the space between the h t, copy and paste => search)
h ttp://www.sigmaaldrich.com/Graphics/Supelco/objects/4800/4709.pdf
h ttp://www.sigmaaldrich.com/Graphics/Supelco/objects/4800/4709.pdf
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