Asked by seth

how many grams of tris and tris Hcl are needed to make up 500ml of 1m tris buffer with a pH of 0.8?

Answers

Answered by DrBob222
I assume you mean 1 M and not 1 m. M is molar; m is molal.
You need two equations.
equation 1 is
pH = pKa + log (base)/(acid)

equation 2 is
(base) + (acid) = 0.8 M

Use equation 1 and solve for (base)/(acid) then (base) = k(acid). Then plug that into equation 1 to find (base) and (acid). Convert molarities into grams per liter , then grams/500 mL.
Post your work if you get stuck.
Answered by Anonymous
Question makes no sense. pH of 0.8 or 8.0????
Answered by Ojole Joseph
pH is 8.0
Answered by Doc48
Here's a reference that's useful for tris systems...(remove the space between the h t, copy and paste => search)
h ttp://www.sigmaaldrich.com/Graphics/Supelco/objects/4800/4709.pdf
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