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kishow
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How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka of Tris is 8.3, mw of Tris is
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Answers (1)
From the question given: Tris Hcl us the Acid,with Mw=157.and then Tris is the base with the Mw=121. then converts 500mls to litre which is 0.5 litre then from the Henderson and Hasselbach equation PH=pka+log(A/H) then 8.0-8.3=log Tri/Tri Hcl then