Here are the answers to your questions regarding the Indian Removal Act (1830):
Question 1: Which of these was NOT one of the "Five Nations" most affected by the Indian Removal Act?
Answer: D. Comanche
Question 2: Per the Indian Removal Act, Native Americans were to be relocated west of the __________.
Answer: C. Mississippi River
Question 3: What was Andrew Jackson's reaction to the Supreme Court decision in Worcester v. Georgia?
Answer: A. He ignored it.