Transferrin (T) -
Molar mass = 81000g/mol - carries 2 Fe3+ ions
Deferrioxxamine B (DB)
molar mass = 650g/mol - carries 1 Fe3+
at wavelength 428
molar absorptivity T = 3240
molar absorptivity DB = 2630
at wavelength 470
molar absorptivity T = 4370
molar absorptivity DB = 2190
A solution of transferrin and desferrioxamine was placed in a cuvet with a path length of
1 cm. The absorbance at 470 nm was 0.42, and the absorbance at 428 nm was 0.40.
Calculate the concentration Fe(III) in transferring, assuming each transferrin binds two
Fe(III)
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I calculated concentration for T at both wavelengths using Beers law and added them to find C=2.19*10^-4M
2.19*10-4 M T * 81000 g/mol = 17.739 g/L T
percent mass of Fe3+ in T is
111.69g/81000g *100%= 0.138%
% Fe3+ in 17.739g T = 0.0245g =4.38*10^-4M Fe3+
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I am not sure if this is correct or if I am even on the right track. There is a second part to the question (which is why I wasn't sure if I needed to use all the information given at the beginning). Any help would be great! thank you
1 answer