The question is below. If someone could at least give me an idea of where to start, or some steps of how I should go about solving this problem, I would be so extremely grateful. Thank you!
You isolate an enzyme from teddy bear liver. The enzyme catalyzes the following reaction: A + B C + D You determine the Km for A to be 3 µM, and for B to be 25 mM. What are the approximate physiological concentrations of A and B in teddy bear cells? The Vmax for A is 2.7 µmoles/min/mg enzyme, what is the Vmax for B? The molecular weight of the enzyme is 40 kD. What is the turnover number (Kcat) for the enzyme? (Units = µmoles of substrate/s/µmole of protein). You have an assay mix containing 1µM A, 100 mM B, and 0.2 mg of enzyme. What is the rate of formation of product under these conditions (B is saturating, A is not) (in µmoles/min/mg enzyme)?