To calculate the concentration of sucrose in the final solution, we need to consider the volume of the final solution and the amount of sucrose present in it.
First, let's calculate the total volume of the final solution by adding up the volumes of all the solutions:
Total Volume = 20 mL (TRIS buffer) + 10 mL (KCl) + 10 mL (sucrose) + 150 mL (dH2O)
Total Volume = 190 mL
Next, let's calculate the amount of sucrose in the final solution by multiplying the concentration of sucrose by its volume:
Amount of Sucrose = 0.30 M (concentration of sucrose) x 10 mL (volume of sucrose)
Amount of Sucrose = 3.00 mmol
Now, we can calculate the concentration of sucrose in the final solution by dividing the amount of sucrose by the total volume:
Concentration of Sucrose = Amount of Sucrose / Total Volume
Concentration of Sucrose = 3.00 mmol / 190 mL
Concentration of Sucrose = 0.0158 M
Therefore, the concentration of sucrose in the final solution is 0.0158 M.
The following solutions are combined:
20 mL of 0.50 M TRIS buffer, pH 7.0
10 mL of 0.25 M KCl
10 mL of 0.30 M sucrose
150 mL dH2O
Calculate the concentration of sucrose in the final solution.
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