The concentration of stain in the bottle was 5% and you added 100 microliters of it to 9.9ml of a buffer to make a working dilution of stain. You then combined 10 microliters of that diluted stain with 40 microliters of your diluted cells and put a few microliters of that mixture on the slide from which you took your photo. The effective (final) concentration of the stain is _____
1 answer
5% x (100/109.9) x (10/50) = ?