Based on the characteristics given in the statements, here's an evaluation of each one:
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Only Plant cells have rough endoplasmic reticulum - This statement is incorrect. Both plant and animal cells have rough endoplasmic reticulum.
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Plants have a larger vacuole - This statement is generally correct. Plant cells typically contain a large central vacuole, which is much larger than those found in animal cells.
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Plant and animal cells have chloroplasts - This statement is incorrect. Chloroplasts are only found in plant cells (and some protists), not in animal cells.
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Animal cells always have cell walls - This statement is incorrect. Animal cells do not have cell walls; they only have a cell membrane.
Given these evaluations, the best response is:
Plants have a larger vacuole.