Once the adsorbent is packed in a liquid chromatography column, it is important that the level of the elution solvent not drop below the top of the adsorbent. Why?

Answer 1

I answered both of these below. If you don't like those answers you need to be more specific about what you want in the answer.

Answer 2

oh no man. i just want different answers to see if the answers are right. And if people agree.

I just don't want to take chances. Do you have a background in chemistry or something? I have no background in chemistry so i don't know if i'm seeing a right answer or not.

Can you elaborate more? like Don't overload the column because ...

Thanks for all your help=]

Answer 3

I have a Ph.D. in chemistry. That Dr in front of Bob222 is real. Why would I answer a question in which I wasn't sure of the answer (but yes, I sometimes makae mistakes)? You are answer shopping. What you are doing is taking a vote on which is the best answer? Basically you are wasting your time and ours. What you should do is look up these answers in your text/notes or look on the web. You can do that yourself and take the best of the lot. You can't believe everything you read on the web but if you go to reliable sites such as Wikipedia and others, the answers can be trusted.