Imagine that your initial absorbable reading for “low salt” fraction was too high, so you diluted your sample 4 times. When you measure the absorbable using 100 ul of the diluted sample , the absorbance is 0.6. What is the concentration of the original undiluted sample (mg/ml). Y=12.235x

Answer 1

To find the concentration of the original undiluted sample, we can use the dilution factor formula.

Dilution factor = initial volume/final volume

Since the sample was diluted 4 times, the dilution factor is 4.

Now, we can calculate the concentration of the original undiluted sample using the formula provided:

Y = 12.235x

Where Y is the absorbance and x is the concentration (in mg/ml).

Given that the absorbance (Y) is 0.6, we can substitute this value into the equation:

0.6 = 12.235x

Now, we can solve for x (the concentration):

x = 0.6/12.235
x ≈ 0.049 mg/ml

However, this concentration is for the diluted sample. To find the concentration of the original undiluted sample, we need to multiply this value by the dilution factor:

Concentration of original undiluted sample = 0.049 mg/ml * 4
Concentration of original undiluted sample ≈ 0.196 mg/ml

Therefore, the concentration of the original undiluted sample is approximately 0.196 mg/ml.