To determine the protein concentration of the flowthrough fraction, we need to use the equation:
y = 12.235x
where y is the absorbance reading and x is the protein concentration (mg/ml).
Given that the absorbance reading is 0.52 and the equation is y = 12.235x, we can rearrange the equation to solve for x:
0.52 = 12.235x
Divide both sides of the equation by 12.235:
0.52/12.235 = x
x = 0.0425
Therefore, the protein concentration of the flowthrough fraction is 0.0425 mg/ml.
You take 0.03 ml of the flowthrough fraction, measure the absorbable using the same conditions as in the standard curve above, and get a reading of 0.52. What is the protein concentration of the flowthrough fraction (mg/ml)? y=12.235x
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