if there is no colonies on the wild type or lysate or ADP-6only plates, did you obtain an approximate 10x-fold change in colony numbers between the different dilutions? did you expect this? pls help

1 answer

Yes, expect that.
in the original plates, it is likely those colonies were dominated by one genotype. See this:
http://www.bio.fsu.edu/courses/mcb4403L/dilution.pdf
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