Asked by Chris

I need to make 10 ml of bacitracin at 1 uM. I have purchased 5 mg of it. The MW of it is 458.6 g. How would I go about doing this and what would be my final answer? Thank you.

concentration= moles/volume= mass/(MW*volume)

or mass= concentration*volume*MW

= 1uM*10ml*458.6g/mole
= 4.586 g *10^-5 or 4.586mg

Put 4.586mg in a vial, add water to make 10 ml of solution, stir, label, and properly dispose of the excess chemical

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