Asked by Courtney
Gas Chromatography:
The internal standard technique is used. A volatile component which does not interfere with the GC analysis (i.e. it gives a separate peak) is added to the sample which is then analyzed. For example, 10.0 mg of D was added to 50.0 mg of the mixture of A, B, and C. The sample is then analyzed by GC (note that you need to inject 1-2 uL) giving a chromatogram with peaks for only A, B, and D. The relative peak areas (i.e. relative to D) are found to be: A, 0.75; B, 3.00; D, 1.00. Fpr 10.0 mg of D, what weights of A and B are indicated by this chromatogram; i.e. what weights of A and B are in the original 50 mg sample? Thus, what are the weight percentages of A and B in the original sample?
I am really confused on how to calculate this, let alone where to even begin. Do you use the equation n= the sum of niWi?
The internal standard technique is used. A volatile component which does not interfere with the GC analysis (i.e. it gives a separate peak) is added to the sample which is then analyzed. For example, 10.0 mg of D was added to 50.0 mg of the mixture of A, B, and C. The sample is then analyzed by GC (note that you need to inject 1-2 uL) giving a chromatogram with peaks for only A, B, and D. The relative peak areas (i.e. relative to D) are found to be: A, 0.75; B, 3.00; D, 1.00. Fpr 10.0 mg of D, what weights of A and B are indicated by this chromatogram; i.e. what weights of A and B are in the original 50 mg sample? Thus, what are the weight percentages of A and B in the original sample?
I am really confused on how to calculate this, let alone where to even begin. Do you use the equation n= the sum of niWi?
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