e
Outcome 2: Assignment
1. Calculate the equilibrium constant for the reaction at pH 7.0 and 25°C
(ΔG°’ = - 9.7 kJ.mol-1
).
Glycerol-3-phosphate + H2O Glycerol + Pi
2. The final step in the pathway for the synthesis of glucose from lactate (gluconeogenesis) is:
Glucose-6-phosphate + H2O Glucose + Pi
When glucose-6-phosphate is incubated with the proper enzyme and the reaction runs until
equilibrium has been reached, the final concentrations are found to be:
glucose-6-phosphate (0.035 mM), glucose (100 mM), and Pi (100 mM).
Calculate ΔG°’ at 25C and pH 7.
3. The Go' for the reaction Citrate Isocitrate is +6.64 kJmol-1
. The Go' for the reaction
Isocitrate α-Ketoglutarate is -267 kJmol-1
.
Calculate the Go' for the conversion of citrate to α-ketoglutarate?
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