Based on the options provided and assuming the chart illustrates the impacts of the Spanish conquest on the Aztec and Incan empires, the best title for the chart would likely be:
"Aztec and Incan empires before and after the Spanish conquest."
This title directly reflects a comparison of the states of these civilizations pre- and post-conquest, which is likely the focus of the chart. The second option, while relevant to broad discussions about colonialism, is less specific to the particular context of the Aztec and Incan empires.