Absorption spectrum refers to the range of wavelength of light that a substance is capable of absorbing. The absorption spectrum is important for studying the pigment and molecules present in the leaves that are responsible for absorbing specific wavelengths of light for photosynthesis. Leaf spectroscopy, measuring spectra of light reflected or absorbed by leaves, is a non-destructive and widely-adopted approached for evaluating the leaf bio-chemical/- physical parameter such as pigment, chlorophyll and area index (Long, 2018). The experience focus on characterized various kinds of leaves and then gradually growing up to various database of different color range. Light absorption was quantified in the following systems: isolated chloroplasts and leaves a widely differing in pigment content, and coleus (Coleus blumei Benth.) at different stages of leaf ontogenesis (Sun, 2019). For these plant species, scattering-compensated absorption spectra of chloroplast suspensions are presented. Comparison of leaf and chloroplast spectra showed considerable changes in the extent of the ‘package’ effect and the lengthening of the effective optical path in a leaf. The difference between leaf and isolated chloroplast absorption could be quantitatively described by adapting Duysens’s treatment of flattening. It was found that the accumulation of chlorophyll in leaves is accompanied by a monotonous enhancement of the package effect (Habibullah M, Mohebian MR, 2015)
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The absorption spectrum is the range of light wavelengths that a substance can absorb, and is crucial for studying the pigments and molecules in leaves responsible for photosynthesis. Leaf spectroscopy, a popular non-destructive method, is used to analyze the biochemical and physical properties of leaves such as pigment and chlorophyll levels. Different types of leaves are examined, along with various color ranges, to assess light absorption. Studies have measured absorption in chloroplasts and leaves with varying pigment levels, as well as at different stages of leaf development. By comparing leaf and chloroplast spectra, researchers have discovered changes in the 'package' effect and effective optical path in leaves. The accumulation of chlorophyll in leaves leads to an increase in the package effect, as described by Duysens’s flattening treatment. This research sheds light on the absorption processes in plants and how they vary based on pigment content and leaf development.