An absorption spectrum is the range of wavelengths of light that a substance can absorb. It is crucial for understanding the pigments and molecules present in leaves that capture specific wavelengths of light for photosynthesis. Leaf spectroscopy, which involves measuring the light reflected or absorbed by leaves, is a popular and non-destructive method for evaluating leaf biochemical and physical parameters such as pigment content and chlorophyll levels.
Research has focused on characterizing different types of leaves and building databases of various color ranges. Studies have quantified light absorption in isolated chloroplasts and leaves with varying pigment content, as well as in coleus plants at different stages of leaf development. By comparing absorption spectra of leaves and chloroplasts, researchers have observed changes in the extent of the 'package effect' and the effective optical path length in leaves. The difference between leaf and isolated chloroplast absorption can be explained by a modification of Duysens's flattening model, indicating that as chlorophyll accumulates in leaves, the 'package effect' strengthens.
Overall, studying absorption spectra in leaves provides valuable insights into the mechanisms of photosynthesis and the role of pigments in capturing light energy. Further research in this area can enhance our understanding of plant biology and contribute to the development of more efficient agricultural and ecological practices.
Absorption spectrum refers to the range of wavelength of light that a substance is capable of absorbing. The absorption spectrum is important for studying the pigment and molecules present in the leaves that are responsible for absorbing specific wavelengths of light for photosynthesis. Leaf spectroscopy, measuring spectra of light reflected or absorbed by leaves, is a non-destructive and widely-adopted approached for evaluating the leaf bio-chemical/- physical parameter such as pigment, chlorophyll and area index (Long, 2018). The experience focus on characterized various kinds of leaves and then gradually growing up to various database of different color range. Light absorption was quantified in the following systems: isolated chloroplasts and leaves a widely differing in pigment content, and coleus (Coleus blumei Benth.) at different stages of leaf ontogenesis (Sun, 2019). For these plant species, scattering-compensated absorption spectra of chloroplast suspensions are presented. Comparison of leaf and chloroplast spectra showed considerable changes in the extent of the ‘package’ effect and the lengthening of the effective optical path in a leaf. The difference between leaf and isolated chloroplast absorption could be quantitatively described by adapting Duysens’s treatment of flattening. It was found that the accumulation of chlorophyll in leaves is accompanied by a monotonous enhancement of the package effect (Habibullah M, Mohebian MR, 2015)
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