A 24-h urine specimen was diluted to 2.000L and buffered to pH 10.0. A 10.00 mL aliquot was titrated with 27.32 mL of 0.003960 M EDTA. The calcium in a second 10.00 mL aliquot was iso;ated as solid CaC2O4, redissolved and titrated with 12.21 mL of the EDTA solution.
a) Calculate the conditional formation constants for complexation of Ca2+ and Mg2+ with EDTA at pH 10.0
b) Assuming that 15-300 mg magnesium and 50-400 mg calcium per day are normal, did this specimen fall within the normal ranges?
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