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A 100 amino acid folded protein (F) is in equilibrium with it’s unfolded (U) (or

denatured) form. Let ΔrH(std) =+347 kJ mol-1 and ΔrS(std) =-1010 J K-1 mol-1 for the equilibrium () at 25℃.

(a) Calculate the concentration of protein in each form if the total
concentration of protein is 10μM.

(b)Assuming that ΔrH(std) is independent of temperature, calculate the equilibrium constant at 37℃ using the van’t Hoff equation.

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