Question
An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated by ammonium sulfate precipitation. The fraction precipitating between 30 and 50% saturation was redissolved in a total volume of 10 mL and dialyzed. The solution after dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction, 20 microliters catalyzed the reaction rate of 5.9 nmol/min under the standard assay conditions. calculate the following:
A. The recovery of enzyme after the ammonium sulfate step.
B. The fold purification after the ammonium sulfate step.
A. The recovery of enzyme after the ammonium sulfate step.
B. The fold purification after the ammonium sulfate step.
Answers
by the way, this question is different from the previous question i asked.
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