Answers by visitors named: stew

which buffer do I choose? There are instructions above the table does this have anything to do with my choice for the problem i am working out, or do I just pick whatever one I want and do the math? Above the table it states, Make two buffers starting with solid material, which is the most common way to make buffers. You will be given a desired pH, and your task is to prepare 100 ml of two appropriate buffers at a concentration of 0.10 M. One of the buffers will be a phosphate or citrate buffer, and the other will be one of the others (not phosphate or citrate). Using the following table, choose the most appropriate buffer compounds for your pHs.

okay, this is what I have so far and now don't know how to finish. I used TRIS. pH=8.30+log(121.1/157.6) pH=8.19 Note: TRIS (Trizma base) MW= 121.1 TRIS-HCL MW= 157.6 (I hope I am using the correct things) After I got the pH, I got stuck on how to finish the problem.

these are pre-lab questions, so i haven't been given a ph yet. These are questions due before lab starts.

okay, let me just stick to the pre-lab question using the table. Here is the pre-lab question: Calculate the weight of the buffers you will use to make the buffers for part A for all the buffer possibilities listed under procedures in part A, stp i. In other words, how many grams do you need to make 100 mL of a 0.1 M buffer? How do I answer this question? I don't know anything about a pH.

Okay, I have worked out the first two. Am I doing them right? For acetate, I got 27.9 grams. For CAPS, I got 5.08 grams. Am I doing these right?

By the way, I have been using a pH of 7 for all of them.

I noticed some mistakes in my conversions. The answer I got for acetate was 37.97 grams. For CAPS, i got.112 grams. Are these correct? Thanks.

okay. Here is the first acid and base worked out. Acetate(base): .3797 grams Acetic acid(acid): .1674 grams Are these right? If they are, i can finish the rest. Thanks.

I have to use molecular weight (60 g/mol) to convert 0.058 M (58nM) to mL. my instructor wrote (M to moles to grams to mL). The answer is 3.34mL I just need to know how to get there.

sorry, just realized i left the density out. The density is 1.049 g/mL. This should help. Thanks.

by the way, this question is different from the previous question i asked.

Note: a Unit is ((ΔA/Δmin)/(6220 M -1 cm -1)) X 106 μM/M X 3 X 10-3 L

I am trying to find x, which is the protein in mg. I know why will represent absorption. What will i put for m and b for my values? I know one axis is y for absorbance, i don't know what other values to put in for the rest of the graph.

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number 2: numerator of third product term should be fact(n), not fact(k)