Question

1) If your spectrophotmeter can measure n absorbance up to 1.5, what is the maximum concentration of NADH that you can measure without diluting?

2) If you add 3 mL of water to 1 mL of NADH, mix and get an absorbance of 0.2, what is the concentration of the original NADH solution?

Thanks!

Answer 1

A = 1.5, then %T = 31.6%. But questions 1 and 2 can't be answered without knowing what the graph looks like OR without knowing the absorptivity constant.

Answer 2

there is no graph or absorbancy constant

Answer 3

does anyone has the answer to this?