You wish to study a variant [a close relative] of the Tobacco Mosaic virus. Specifically you want to characterize the capsid and genetic component as well as determine the viral load required to cause a disease state.

Question

a. What methodologies might you use to isolate and characterize this microbe?

b. How will you determine the LD50 of this viral particle?

I have been searching the net, but I am not having much luck. I have found a few things on the LD50, such as inoculating mice maybe, but nothing on part a. Any help will be greatly appreciated.

Answer 1

Since Jiskha doesn't have any regular biology experts, I suggest you post your question at this biology forum.

http://biology-forums.com/index.php