Question

When trying to purify a protein using column chromatography, what happens to the mass of the protein eluted from the column if you accidentally run it dry? Would it be more or less? Thanks.

Answer 1

My best guess is that it would be less. If the column runs dry the protein sticks to the stationary phase, and you have no assurance that (a)the protein will re-enter the mobile phase in its entirety, and (b) that no degradation of the protein will occur.