In "Star Wars: Episode III – Revenge of the Sith," Darth Vader, formerly Anakin Skywalker, does have a tumultuous and emotional encounter with Obi-Wan Kenobi on Mustafar. However, there isn’t a direct scene in which Vader questions Obi-Wan about his children or Padmé during their duel.
The significant emotional aspect of their confrontation is rooted in betrayal, loss, and the consequences of Anakin's decisions. Anakin has fallen to the dark side, believing that his new power will help him save Padmé. In their fight, Obi-Wan confronts Anakin’s transformation and expresses sorrow for his former apprentice's choices and the path he has taken.
If you’re referring to emotional themes or implications regarding Anakin’s relationship with Padmé and the existence of their children, those are more deeply explored in the surrounding narrative than in this particular duel. Would you like more detailed information about their relationship or the events leading up to their duel?