Question

HEPES, is a commonly used component of physiological buffers. The PKa of the protonated amino group of HEPES is 7.55 at 25C.

Describe the preparation of 1.00L of 0.100M HEPES buffer, pH 8.00, from the sodium salt of the basic form of HEPES (MW 260.3), .0M HCL and deionized water

Answer 1

You need to read your posts to make sure they are ok. This post omits the concn HCl.

Answer 2

The conc is 6.0M HCL