The item that does not belong in the group is "Trust" because the other three items—"Guilt," "Sleep," and "Macbeth"—are directly connected to the themes of ambition and moral conflict in Shakespeare's play, while "Trust" does not prominently exemplify these themes.
In "Macbeth," the theme of guilt is central as Macbeth wrestles with the consequences of his murderous ambition; for instance, he famously states, "Methought I heard a voice cry, 'Sleep no more! Macbeth does murder sleep,'" highlighting the psychological turmoil he faces after committing regicide. Sleep itself serves as a metaphor for innocence and peace of mind, which is shattered by Macbeth’s actions. Furthermore, Macbeth's inability to sleep reflects his overwhelming guilt, leading to a descent into madness, as seen in Lady Macbeth's compulsive hand-washing in her futile attempt to cleanse herself of guilt: "Out, damned spot!" Together, these elements illustrate the profound effects of ambition, guilt, and the loss of peace, which are far more pivotal to the narrative than the concept of trust.