Firstly, collected 5kg leaves of Tithomia.diversifolia, air dried them preferably leaves for five to seven days as per the advised from a lecture. After drying, then gathered them together and chopped all the leaves with the aid of scissors. Then did a quality check to ensure pure and quality leaves were to put into blender for blending.

Question

Secondly, extraction phase, open the blender placed the first hips of Tithonia divorsifolta air dried leaves cuttings. After placing, the lid of the juice blender tightly closed. After that plugged the blender to the power point. Then put on the switch on and also pressed the on button switch of blender to start blending. Several times a student blended the leaves for 3 to 5 minutes. As the blending process was observed closely and all the dried leaves turned into grey powder or dust. Then put off the power switch and poured all the powder of Mary gold leaves into paper tissue and preserved it aside. Thus then did it for the second hips of Mexican dried leaves, followed the same processes as done at the first place.
Initially poured the grinded grey powder on the paper tissue, and then placed it on the balance to get the exact mass of 20g powder into 200ml dichloromethane and 200ml ethanol. Soon after that rapped them with foil. Then labelled those both 200ml beakers contained of dichloromethane and ethanol. After labelling preserved them for 3-4 weeks for absorption before extraction of using dichloromethane and chloroform.
Thirdly, the evaporation process began right after 4 weeks. Initially placed the mixture contained with dichloromethane and Tithonia diversifiolic on the heater or heating plate to obtain the Antibacterial Activity of Crude Aqueous Extracts of Tithonia diversifolia from Zocki Zoi Area, Goroka, EHP.
Concentration or crude extract using rotary evaporating iron. While liquid extracted was on evaporation process, prepared the disk preparation for the inhibition of growth of microorganism and then used the test compounds in agar plate.
The fourth or last phase of the experiment was the Antimicrobial Test. Prepared the bacterial culture which was done through pour plate method Prepared a serial dilution of mixture sample. In that process, sterilized the inoculating loop, and then removed the cap from tubes very carefully. Then flamed the lip of the tube and placed sterile portion of inoculating loop into broth, then removed. Flamed the lip of the tube and replaced the cap. Then genteelly streaked the surface of an agar plate with the inoculating loop or used by swabs.

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Answer 1

Firstly, I collected 5kg of Tithonia diversifolia leaves and air dried them for five to seven days as recommended during a lecture. After drying, I gathered the leaves and used scissors to chop them finely. I then conducted a quality check to ensure only pure, high-quality leaves were selected for blending.

In the extraction phase, I opened the blender and placed the first batch of air-dried Tithonia diversifolia leaf cuttings inside. After securely fastening the lid, I plugged in the blender and turned it on, blending the leaves for 3 to 5 minutes. I closely monitored the process until all the dried leaves were transformed into a grey powder. Once blending was complete, I switched off the power and transferred the leaf powder onto a paper tissue, setting it aside. I repeated this process for the second batch of dried leaves.

Next, I measured the ground grey powder. I placed the powder on a balance, ensuring an exact mass of 20g, and then dissolved it into 200ml of dichloromethane and 200ml of ethanol. I wrapped the solutions in foil and labeled the beakers accordingly. These beakers were then preserved for 3 to 4 weeks to allow for absorption before extracting with dichloromethane and chloroform.

After 4 weeks, the evaporation process began. I placed the mixture containing dichloromethane and Tithonia diversifolia on a heater or heating plate to obtain the antibacterial activity of the crude aqueous extracts of Tithonia diversifolia from the Zocki Zoi area in Goroka, EHP. The concentration or crude extract was processed using rotary evaporating iron. While the liquid extract was evaporating, I prepared discs for testing the inhibition of microbial growth and then used the test compounds on agar plates.

The final phase of the experiment was the antimicrobial test. I prepared the bacterial culture using the pour plate method and performed serial dilutions of the mixture sample. To avoid contamination, I sterilized the inoculating loop, carefully removed the caps from the tubes, flamed the lip of each tube, dipped the sterile loop into the broth, and then removed it. I flamed the lip of the tube again before replacing the cap. Lastly, I gently streaked the surface of an agar plate with the inoculating loop or used swabs.