Firstly, collected 5kg leaves of Tithomia.diversifolia, air dried them preferably leaves for five to seven days as per the advised from a lecture. After drying, then gathered them together and chopped all the leaves with the aid of scissors. Then did a quality check to ensure pure and quality leaves were to put into blender for blending.

Question

Secondly, extraction phase, open the blender placed the first hips of Tithonia divorsifolta air dried leaves cuttings. After placing, the lid of the juice blender tightly closed. After that plugged the blender to the power point. Then put on the switch on and also pressed the on button switch of blender to start blending. Several times a student blended the leaves for 3 to 5 minutes. As the blending process was observed closely and all the dried leaves turned into grey powder or dust. Then put off the power switch and poured all the powder of Mary gold leaves into paper tissue and preserved it aside. Thus then did it for the second hips of Mexican dried leaves, followed the same processes as done at the first place.
Initially poured the grinded grey powder on the paper tissue, and then placed it on the balance to get the exact mass of 20g powder into 200ml dichloromethane and 200ml ethanol. Soon after that rapped them with foil. Then labelled those both 200ml beakers contained of dichloromethane and ethanol. After labelling preserved them for 3-4 weeks for absorption before extraction of using dichloromethane and chloroform.
Thirdly, the evaporation process began right after 4 weeks. Initially placed the mixture contained with dichloromethane and Tithonia diversifiolic on the heater or heating plate to obtain the Antibacterial Activity of Crude Aqueous Extracts of Tithonia diversifolia from Zocki Zoi Area, Goroka, EHP.
Concentration or crude extract using rotary evaporating iron. While liquid extracted was on evaporation process, prepared the disk preparation for the inhibition of growth of microorganism and then used the test compounds in agar plate.
The fourth or last phase of the experiment was the Antimicrobial Test. Prepared the bacterial culture which was done through pour plate method Prepared a serial dilution of mixture sample. In that process, sterilized the inoculating loop, and then removed the cap from tubes very carefully. Then flamed the lip of the tube and placed sterile portion of inoculating loop into broth, then removed. Flamed the lip of the tube and replaced the cap. Then gently streaked the surface of an agar plate with the inoculating loop or used by swabs.
correct the grammar for the above information.

Answer 1

Firstly, I collected 5 kg of Tithonia diversifolia leaves and air-dried them for five to seven days, as advised in a lecture. After drying, I gathered the leaves together and chopped them using scissors. I then performed a quality check to ensure pure and high-quality leaves before placing them into a blender for blending.

Secondly, during the extraction phase, I opened the blender and placed the first batch of Tithonia diversifolia air-dried leaf cuttings inside. After placing the leaves in the blender, I securely fastened the blender lid. Then, I plugged the blender into a power point, switched it on, and pressed the "on" button to start the blending process. I blended the leaves for 3 to 5 minutes, observing closely until all the dried leaves turned into a grey powder. I then turned off the power switch and poured the powder onto a paper tissue for later use. I repeated this process for the second batch of Mexican dried leaves.

Initially, I poured the ground grey powder onto a paper tissue and then placed it on a balance to measure exactly 20 g of powder. I added this to 200 ml of dichloromethane and 200 ml of ethanol. I wrapped the mixtures with foil and labeled the beakers accordingly. These beakers were then preserved for 3-4 weeks to allow for absorption before extraction using dichloromethane and chloroform.

Thirdly, the evaporation process began after 4 weeks. I placed the mixture containing dichloromethane and Tithonia diversifolia on a heater or heating plate to obtain the antibacterial activity of the crude aqueous extracts of Tithonia diversifolia from Zocki Zoi Area, Goroka, EHP. The concentration or crude extract was prepared using a rotary evaporator. While the liquid extract was evaporating, I prepared disks for testing the inhibition of microorganism growth and tested the compounds on an agar plate.

The fourth and final phase of the experiment was the antimicrobial test. I prepared the bacterial culture using the pour plate method and made a serial dilution of the mixture samples. During this process, I sterilized the inoculating loop, carefully removed the caps from the tubes, flamed the lip of each tube, placed the sterile portion of the inoculating loop into the broth, and removed it. I flamed the lip of the tube again and replaced the cap. Then, I gently streaked the surface of an agar plate with the inoculating loop or used sterile swabs.