Firstly prepare an extract of chlorophyll by weight 0.3 grams of the different colored coleus and then tear the leaf section into small pieces and place the tissue in a mortar for grinding. Then add 10mL of ethanol to the sample and grand with the pestle until only tiny specks of the leaf tissue remain, add an additional of 20 mL of ethanol to the mortar. The extract was then filtrated using filter paper in a filter funnel into beaker, the extract solution then pipetted into a cuvette. The extract must fall into a specified range to provide a reading with the spectrophotometer. The wavelength set on the spectrophotometer was 400 nm using solvent cuvette, measurement taken was at 400 nm and recorded in a table. These steps were repeated using wavelengths up to 400 nm and 700 nm results was recorded in the table. After that the result was used to create a graph of an absorption spectrum.

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Answer 1

To start, extract chlorophyll by grinding 0.3 grams of variously colored coleus leaves in a mortar with 10mL of ethanol. Add an additional 20mL of ethanol to the mixture and filter the extract using filter paper. Transfer the extract solution to a cuvette and measure the absorbance at various wavelengths ranging from 400 nm to 700 nm using a spectrophotometer. Record the measurements in a table and use the data to create an absorption spectrum graph.