difference between dideoxyribose and deoxyribose

Answer 1

Dideoxyribose is a modified sugar that lacks a hydroxyl (-OH) group on its 3' carbon. This is important in DNA sequencing because when a nucleotide containing dideoxyribose is incorporated into a growing DNA chain, it cannot form a phosphodiester bond with the next nucleotide, which terminates chain elongation.

Deoxyribose is a sugar found in DNA that has a hydroxyl (-OH) group on its 3' carbon, which allows for continued chain elongation during DNA synthesis. It is similar to ribose, the sugar found in RNA, but lacks an additional hydroxyl group on its 2' carbon.