How to calculate the percentage of vitamin C in cereal B

Answer 1

To calculate the percentage of vitamin C in cereal B, you will need to perform a vitamin C assay. Here are the steps:

1. Weigh a known amount of cereal B (e.g. 10 grams) and grind it into a fine powder.
2. Add a known amount of a reducing agent (e.g. 2,6-dichlorophenolindophenol or DCPIP) to the cereal powder and mix well.
3. Add a known amount of a vitamin C standard solution to the cereal powder and mix well.
4. Titrate the mixture with a standardized solution of iodine until the DCPIP changes color from blue to colorless.
5. Repeat steps 3 and 4 with the cereal sample instead of the vitamin C standard solution.
6. Calculate the amount of vitamin C in the cereal sample using the formula:

Vitamin C (mg/100g) = (V2 - V1) x C x 100 / W

where V1 is the volume of iodine solution used for the blank, V2 is the volume of iodine solution used for the cereal sample, C is the concentration of iodine solution (in mg/mL), and W is the weight of the cereal sample (in grams).

7. Finally, calculate the percentage of vitamin C in cereal B by dividing the amount of vitamin C by the weight of the cereal sample and multiplying by 100.