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To find the sample volume, we need to subtract the initial water level of 20.00 mL from the final water level of 28.50 mL.
So, the sample volume = final water level - initial water level
Sample volume = 28.50 mL - 20.00 mL
Sample volume = 8.50 mL
The answer to the question is 8.50 mL.
Since the initial water level is measured to 2 significant figures (20.00 mL), we should report the answer to the same number of significant figures.
Therefore, the correct answer is 8.50 mL.