b) BclI cleaves after the first T:
3’ TGATCA 5’
5’ ACTAGT 3’
Does cleavage by BclI result in a 5’ or 3’ overhang? What is the sequence of this overhang? [1]
3’ TGATCA 5’
5’ ACTAGT 3’
Does cleavage by BclI result in a 5’ or 3’ overhang? What is the sequence of this overhang? [1]