25mg of glycogen is supplied – what is its’ percentage purity?

Question

The glycogen sample was hydrolysed to glucose monomers with 2ml of 2M sulphuric acid in water. The hydrosylate was then neutralised and made up to 10ml total volume. The glucose content was assayed enzymatically and the results gave a content of 2.35mg/ml of glucose. Remember glycogen is a polymer of glucose made via a condensation reaction between glucose monomers losing water during the condensation and that hydrolysis reverses this process.

Answer 1

In the 10 mL sample you have 2.35 mg/mL. So in 10 mL you have 2.35 x 10 = 23.5 mg. The sample was 25 to start. 23.5/25.0 is what percentage.