Question

An enzyme sample contains 24 mg protein per mL. Twenty µL of this sample in a standard incubation volume of 0.1 mL catalysed the conversion of substrate X to product Y at a rate of 1.6 nmol per minute. Calculate the velocity in terms of:
a) µmol per minute
b) µmol per litre per minute
c) µmol per mg protein per minute

Answer 1

a) 1.6 µmol per minute
b) 16 µmol per litre per minute
c) 0.067 µmol per mg protein per minute