Question

A serial dilution is set up with an initial antigen concentration of 250 ug/mL. A serial dilution is carried out where 20 uL is moved from each tube into an adjacent which has been prefilled with 80 uL of buffer. What is the tube concentration of tube 8, again assuming that tube 1 has a concentration of 250 ug/mL?
7 steps from 1 to 8 ... each step is 1/5 as concentrated as the previous one

250 * (1/5)^7

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