Question

Calculate the final concentration of ONPG (in μM) if you add 6.16 mL of 3.3 μM ONPG and dilute to a final volume of 10 mL with PBS buffer.

I thought you would use M1V1 = M2V2 however it came up wrong

I know we are adding 3.84 mL of PBS to the 6.16 mL of ONPG to make a final volume of 10 mL, however, I have no clue how to find the final concentration.

Any help is greatly appreciated