Question

You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1.
What is the concentration of the components (Tris and Tris-H+) in the
final buffer solution?
Use the Henderson-Hasselbalch equation. That is equation 1.
7.5 = 8.1 + log (tris)/(tris-HCl)

equation 2 is:
tris + tris-HCl = 50 mM

Solve the two equations simultaneously to determine tris and trish-H^+

Related Questions

Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M T... How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1)... How many gramms of Tris and Tris Hcl are needed to make up 500ml of 1m Tris with a PH of 8.0? (pka o... how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of...