Asked by silvia
if there is no colonies on the wild type or lysate or ADP-6only plates, did you obtain an approximate 10x-fold change in colony numbers between the different dilutions? did you expect this? pls help
Answers
Answered by
bobpursley
Yes, expect that.
in the original plates, it is likely those colonies were dominated by one genotype. See this:
http://www.bio.fsu.edu/courses/mcb4403L/dilution.pdf
in the original plates, it is likely those colonies were dominated by one genotype. See this:
http://www.bio.fsu.edu/courses/mcb4403L/dilution.pdf
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