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Question: A subtilisin with an active site mutation of His t...
EDIT QUESTION
A subtilisin with an active site mutation of His to Ala had a substantially decreased rate of reaction with the substrate Phe-Ala-Gln-Phe-X (cleavage site between Phe and X). Which of the following would have a reaction rate similar to native subtilisin?
A.
His-Ala-Gln-Phe-X
B.
Phe-His-Gln-Phe-X
C.
Phe-Ala-His-Phe-X
D.
Phe-Ala-Gln-His-X
E.
None of the answers is correct.
Would the answer for this one be D?